Composite

Part:BBa_K4475008

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-09-29)


CTB3 Constitutive Weak Protein Generator Yeast

Construct designed for weak expression of the fungal enzyme CTB3 in saccharomyces cerevisiae, utilizing the Yeast STE5 Promoter and CYC100 Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1864
    Illegal XbaI site found at 93
    Illegal XbaI site found at 701
    Illegal XbaI site found at 1058
    Illegal XbaI site found at 1526
    Illegal XbaI site found at 1844
    Illegal XbaI site found at 1982
    Illegal XbaI site found at 2252
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1864
    Illegal BglII site found at 989
    Illegal BglII site found at 1163
    Illegal BglII site found at 1892
    Illegal BglII site found at 3164
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1864
    Illegal XbaI site found at 93
    Illegal XbaI site found at 701
    Illegal XbaI site found at 1058
    Illegal XbaI site found at 1526
    Illegal XbaI site found at 1844
    Illegal XbaI site found at 1982
    Illegal XbaI site found at 2252
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1864
    Illegal XbaI site found at 93
    Illegal XbaI site found at 701
    Illegal XbaI site found at 1058
    Illegal XbaI site found at 1526
    Illegal XbaI site found at 1844
    Illegal XbaI site found at 1982
    Illegal XbaI site found at 2252
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None